Coding
FL PbEL04

Part:BBa_K4139012:Design

Designed by: Zachary Robinson & Jason Luddu   Group: iGEM22_Lethbridge   (2023-09-26)


Full Length PbEL04


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 105
    Illegal PstI site found at 231
    Illegal PstI site found at 354
    Illegal PstI site found at 477
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 105
    Illegal PstI site found at 231
    Illegal PstI site found at 354
    Illegal PstI site found at 477
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 105
    Illegal PstI site found at 231
    Illegal PstI site found at 354
    Illegal PstI site found at 477
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 105
    Illegal PstI site found at 231
    Illegal PstI site found at 354
    Illegal PstI site found at 477
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted a full length version of PbEL04 so that we can test our antibody with the proteins seen in field. We added a His-tag at the end to assist with purification.

Source

Plasmodiophora brassicae

References

Jiang, X., Su, Y., & Wang, M. (2022). A small cysteine-rich protein identified from the Proteome of clubroot pathogen, Plasmodiophora brassicae, induces cell death in nonhost plants and host plants. https://doi.org/10.21203/rs.3.rs-1961445/v1